Here's a worksheet to practice your skills with gene regulation in prokaryotes using the lac operon.
You can download the worksheet as a Word document online.
Here are the solutions! You can make them larger by going right to YouTube (click its icon at the lower-right side of each video). There is one video for each of the three stages above: Basic, with a Twist, and Sophisticated. Basic
Here's a chance for you to try your hand at gene expression. In this exercise, you're given a piece of DNA and you're told that it encodes an mRNA that has a single intron. You need to transcribe and translate it, and you also need to use protein information to determine where the intron is.
You can access this question in a Word document online.
Find the intron:
You’ve
cloned and sequenced a piece of DNA and you know that it contains a gene for
the protein you’ve been working to characterize. The protein contains the amino acid sequence:
Write out
the full pre-mRNA, including identifying the ends.
Assume that your sequenced DNA would have had the promoter to the left
and that the first nucleotide is at the -3 position. Circle the intron in your mRNA. Write out the full polypeptide, and label its
ends as well.
Try the question yourself before accessing the YouTube solution below:
(You can get a larger image by clicking on the YouTube logo in the bottom-right hand corner of the video above).
